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2 edition of Structural, mutational and kinetic analyses of Aquifex aeolicus prephenate dehydrogenase: Elucidation of the catalytic mechanism. found in the catalog.

Structural, mutational and kinetic analyses of Aquifex aeolicus prephenate dehydrogenase: Elucidation of the catalytic mechanism.

Warren Da-Ren Sun

Structural, mutational and kinetic analyses of Aquifex aeolicus prephenate dehydrogenase: Elucidation of the catalytic mechanism.

by Warren Da-Ren Sun

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Published .
Written in English


About the Edition

Using the crystal structures as guides, active site residues were targeted for site-directed mutagenesis. Kinetic analyses of these variants combined with structural analyses indicate that Ser126 facilitates catalysis by coordinating the prephenate-His147-NAD+ interaction and hydrogen bonding to prephenate via the 4-hydroxyl group, while a gated mechanism consisting of Glu153-Arg250-Asp" modulates substrate access to the active site.Prephenate dehydrogenase, a member of the TyrA family, participates in tyrosine biosynthesis by catalyzing the oxidative decarboxylation of prephenate to 4-hydroxyphenylpyruvate in the presence of NAD+. The current study attempts to elucidate the catalytic mechanism of this enzyme through structural and mutational approaches.Aquifex aeolicus prephenate dehydrogenase was co-crystallized with NAD+, NAD+ and tyrosine, and NAD + and 4-hydroxyphenylpropionate. These crystal structures provide positional information on known active site residues and reveal the interactions between enzyme and substrate.The results presented from this study provide a framework for understanding the mechanism of substrate discrimination within the TyrA family.

The Physical Object
Pagination104 leaves.
Number of Pages104
ID Numbers
Open LibraryOL19551566M
ISBN 109780494211656


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Structural, mutational and kinetic analyses of Aquifex aeolicus prephenate dehydrogenase: Elucidation of the catalytic mechanism by Warren Da-Ren Sun Download PDF EPUB FB2